Research Information System
İPEK D. (Executive), DEMİREL ZORBA N. N.
Project Supported by Higher Education Institutions, 2018 - 2019
Quorum
Sensing (QS) is defined as a chemical communication system of microorganisms
which can affect biofilm formation, virulence, sporulation, motility and toxin
production. Biofilm formation on process line surfaces is an important problem
for food industry, because of cross-contaminations. In this study, we aimed to
determine QS signal presence of real process line isolated, biofilm positive
isolates. Long-chain signals (C15-HSL, C16-HSL and C18-HSL) of
biofilm-positive, gram-negative isolates which none of AHL signal presence were
determined by using indicator microorganisms and HPLC method; besides DFD
(4,5-dihidroksi-2,3-pentadione) AI-2 signals of biofilm-positive, gram-positive
and AI-2 active isolates were studied by using LC/MSMS chromatography methods.
Long-chain AHLs and DFD signals were determined from isolates. These results
demonstrated that quorum sensing system has an important role for biofilm
formation, bacterial communication system is a complex system and indicator
microorganism method may not be enough to define these signals presence.