Gıda Üretim Hatlarından İzole edilen Biyofilm Pozitif Gram Negatif ve Pozitif İzolatların Quorum Sensing Sinyal Varlığının LCMSMS ile Araştırılması


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İPEK D. (Executive), DEMİREL ZORBA N. N.

Project Supported by Higher Education Institutions, 2018 - 2019

  • Project Type: Project Supported by Higher Education Institutions
  • Begin Date: April 2018
  • End Date: March 2019

Project Abstract

Quorum Sensing (QS) is defined as a chemical communication system of microorganisms which can affect biofilm formation, virulence, sporulation, motility and toxin production. Biofilm formation on process line surfaces is an important problem for food industry, because of cross-contaminations. In this study, we aimed to determine QS signal presence of real process line isolated, biofilm positive isolates. Long-chain signals (C15-HSL, C16-HSL and C18-HSL) of biofilm-positive, gram-negative isolates which none of AHL signal presence were determined by using indicator microorganisms and HPLC method; besides DFD (4,5-dihidroksi-2,3-pentadione) AI-2 signals of biofilm-positive, gram-positive and AI-2 active isolates were studied by using LC/MSMS chromatography methods. Long-chain AHLs and DFD signals were determined from isolates. These results demonstrated that quorum sensing system has an important role for biofilm formation, bacterial communication system is a complex system and indicator microorganism method may not be enough to define these signals presence.