Development of ultra-sensitive and selective molecularly imprinted polymer-based electrochemical sensor for L-lactate detection

Piskin E., Cetinkaya A., Eryaman Z., Karadurmus L., ÜNAL M. A., SEZGİNTÜRK M. K., ...More

Microchemical Journal, vol.204, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 204
  • Publication Date: 2024
  • Doi Number: 10.1016/j.microc.2024.111163
  • Journal Name: Microchemical Journal
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Keywords: L-lactate, Molecularly imprinted polymers, Nanoparticle, Photopolymerization, Voltammetry
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Lactate detection is important for the food and healthcare industries, and it's especially important when there's tissue hypoxia, hepatic illness, bleeding, respiratory failure, or sepsis. A new molecularly imprinted polymer (MIP) based electrochemical sensor was fabricated for differential pulse voltammetric assay of L-lactate (LAC). By using ZIF-8@ZnQ nanoparticles, the number of regions and effective surface area were increased. The polymeric film was obtained using 4 aminobenzoic acid (4-ABA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, 2-hydroxyethyl methacrylate (HEMA) as basic monomers, and 2-hydroxy-2-methylpropiophenone one as initiator. The developed 4-ABA/LAC/ZIF-8@ZnQ@MIP-GCE was morphologically characterised using SEM and electrochemically using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements. A linear range of 0.1–1.0 pM LAC with a detection limit of 29.9 fM was found. Lastly, the MIP-based electrochemical sensor detected LAC in commercial human serum samples.