Two biosensors for phenolic compounds based on mushroom (Agaricus bisporus) homogenate: Comparison in terms of some important parameters of the biosensors

Teke M., Sezgintuerk M. K., DİNÇKAYA E., Telefoncu A.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, vol.38, no.1, pp.51-60, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 1
  • Publication Date: 2008
  • Doi Number: 10.1080/10826060701774346
  • Journal Name: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.51-60
  • Keywords: enzyme based biosensors, polyphenol oxidase, biosensor stability, bioimprinted biosensors, GLUCOSE-OXIDASE, CROSS-LINKING
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other.