THERAPEUTIC POTENTIAL OF OZONE AND L-CARNITINE COMBINED ADMINISTRATIONS AGAINST EXPERIMENTALLY-INDUCED ACETAMINOPHEN


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Paldır E., Eroğlu H. A.

Journal Of Animal And Plant Sciences, cilt.31, ss.981-987, 2021 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31
  • Basım Tarihi: 2021
  • Doi Numarası: 10.36899/japs.2021.5.0331
  • Dergi Adı: Journal Of Animal And Plant Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Business Source Elite, Business Source Premier, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.981-987
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Acetaminophen is a widely used medicine with antipyretic and analgesic effects. N-acetyl-P-benzoquinoneimine accumulation after a high-dose of acetaminophen leads to hepatotoxicity, depletion of glutathione stores, and suppression of the antioxidant defense mechanism. As a result, N-acetyl-P-benzoquinoneimine cannot be eliminated from the body and hepatotoxicity occurs. In this study, the effects of the separate and combined use of ozone and L-carnitine in high- dose acetaminophen induced hepatotoxicity was investigated. A total of 56 female Wistar albino rats were randomly divided into 8 groups of seven rats. Acetaminophen was administered orally as a single dose to induce liver damage, and 0.5 mg/kg of 95% oxygen plus 5% ozone gas mixture was administered intraperitoneally. After 1-hour APAP administration, L-carnitine were given 500 mg/kg by intraperitoneally. Serum and tissue oxidant/antioxidant parameters were measured to deduce their combined effect. ANOVA and Tukey’s multiple comparison test were used for statistical analysis. Acetaminophen+L-carnitine+ozone administration caused a significant decrease in the levels of serum malondialdehyde, total oxidant level, aspartate aminotransferase, alanine aminotransferase, and tissue malondialdehyde, total oxidant status levels, while it led to a significant increase in the levels of tissue and serum total antioxidant levels. Nonetheless, a clear evidence of superiority over the sole use of ozone or L-carnitine in acetaminophen induced hepatotoxicity was not present. It was concluded that ozone, L-carnitine and ozone+L-carnitine treatments in acetaminophen induced hepatotoxicity protected the organism against the harmful effects of free radicals and activated the antioxidant mechanism by suppressing oxidative stress.