Fabrication of Electrochemical Immunosensor for Detection of Interleukin 8 Biomarker via Layer-by-layer Self-assembly Process on Cost-effective Fluorine Tin Oxide Electrode

AYDIN E. B., SEZGİNTÜRK M. K.

ELECTROANALYSIS, vol.33, no.6, pp.1596-1605, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1002/elan.202060603
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.1596-1605
  • Keywords: 3-(triethoxysilyl)propyl isocyanate, Disposable electrode, Fluorine tin oxide, Interleukin 8
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

A highly sensitive impedimetric immunosensor for the determination of interleukin 8 (IL 8) cancer biomarker was fabricated via simple fabrication process. A silane agent with isocyanate groups, 3-(triethoxysilyl)propyl isocyanate (IPTES), were utilized for binding of IL 8 antigen specific antibodies and self-assembled on the fluorine tin oxide (FTO) substrate with a standard silanization chemistry. Under optimum experimental conditions, the designed immunosensor had a wide linear detection range of 0.02-4 pg/mL, a low limit of detection of 11.9 fg/mL and a high sensitivity of 4.42 k omega pg(-1) mL cm(-2). The designed biosensor displayed high reproducibility and repeatability, good selectivity against interference biomarkers.