A bio-imprinted urease biosensor: Improved thermal and operational stabilities

Teke M., Sezginturk M. K., Dinçkaya E., Telefoncu A.

TALANTA, vol.74, no.4, pp.661-665, 2008 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 4
  • Publication Date: 2008
  • Doi Number: 10.1016/j.talanta.2007.06.031
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.661-665
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Despite the increasing number of applications of biosensors in many fields, the construction of a steady biosensor remains still challenging. The high stability of molecularly bio-imprinted enzymes for its substrate can make them ideal alternatives as recognition elements for sensors. Urease (urea aminohydrolase, EC 3.5.1.5), which catalysis the hydrolysis of urea to ammonia and carbon dioxide, has been used in immobilized form in artificial kidney for blood detoxification. According to one report approximately half a million patients worldwide are being supported by haemodialysis.