An electrochemical immunosensor based on graphite paper electrodes for the sensitive detection of creatine kinase in actual samples


DEMİRBAKAN B., SEZGİNTÜRK M. K.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, vol.921, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 921
  • Publication Date: 2022
  • Doi Number: 10.1016/j.jelechem.2022.116656
  • Journal Name: JOURNAL OF ELECTROANALYTICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Keywords: Graphite paper (GP), Creatine kinase (CK), Biosensor, Cardiovascular disease, ACUTE MYOCARDIAL-INFARCTION, SELF-ASSEMBLED MONOLAYERS, GOLD NANOPARTICLES, DIAGNOSIS, SURFACE

Abstract

The enzyme creatine kinase (CK) is one of the most well-established biomarkers in cardiovascular disease. For acute myocardial infarction (AMI) diagnosis, CK has a clinical comorbidity rate of 90%. This study has developed a novel electrochemical immunosensor using disposable graphite paper electrodes (GP). The GP electrodes were modified with gold nanoparticles (AuNP) to facilitate CK detection. Afterwards, GP electrodes were covalently immobilized with 6-mercapto-1-hexanol (6-MH), resulting in self-assembled monolayers (SAMs). Then, the electrodes were formed with a 3-Glycidyloxypropyltrimethoxysilane (3-GOPE) agent. Afterwards, the electrodes were immobilized with anti-CK (antibody creatine kinase) protein. In the final immobilization step, BSA (bovine serum albumin) protein was used to block non-covalent interactions.