Akademik Veri Yönetim Sistemi
Chron Precis Med Res , cilt.3, sa.3, ss.107-114, 2022 (Hakemli Dergi)
Aim: Natural products such as honey, pollen, propolis and royal jelly are generally friendly to the human liver and all other body cells, with a high antioxidant capacity. The present study aims at observing the damages caused by Aflatoxin B1 on rat liver and determining the beneficial effects of propolis on liver tissue.
Material and Method: The duration of the study was determined as 60 days and 45 male rats of Wistar albino breed with an average weight of 250-350 grams suitable for the working conditions were included in the study. Experimental animals were randomly and equally divided into 3 groups (The Control Group, The Aflatoxin B1 group, The Aflatoxin B1 + IDA Propolis group). The rats liver tissue and blood samples were taken and placed in separate sterile vials. These liver tissues and blood samples were sent to the our hospital Pathology and
Biochemistry Department for histopathological examination and for biochemical blood analysis to be performed.
Result: The Gamma glutamyl transferase (GGT), Alanine Aminotransferase (ALT) and Alkaline phosphatase (ALP) levels of groups that were administered Aflatoxin B1 and Aflatoxin B1 + Propolis were higher than those of the control group. Administering Aflatoxin B1 alone increased Aspartate Aminotransferase (AST) levels whereas administering Aflatoxin B1 + Propolis decreased AST levels.
The Total Bilirubin, Direct Bilirubin and c-reaktive protein (CRP) levels of groups that were administered Aflatoxin B1 and Aflatoxin B1 + Propolis were higher than those of the control group. The Total Bilirubin, Direct Bilirubin and CRP levels of rats that were administered Aflatoxin B1 were higher than those of rats that were administered Aflatoxin B1 + Propolis. Administering Aflatoxin B1 and Aflatoxin B1 + Propolis have increased the malondialdehyde (MDA) levels but decreased the catalase (CAT) levels, and this decrease is statistically significant (p<.0001). Administering Aflatoxin B1 + Propolis has resulted in a similar level of glutathione peroxidase.(GPx) with the control group. However, administering Aflatoxin B1 alone has resulted in a GPx level that is lower than both other groups. Administering Aflatoxin B1 and Aflatoxin B1 + Propolis have caused a statistically significant difference in the total antioxidant (TAS), total oxidant (TOS) and superoxide dismutase (SOD) levels (p<.0001).
Conclusion: The results of this study indicate that bee pollen is an important source of flavonoids, which can be considered as natural antioxidants. It is also reported that bee products prevent lipid peroxidation and scavenge a number of free oxygen radicals that are known oxidants and carcinogenic agents.
Keywords: Aflatoxin B1, histopathology, hepatic antioxidant defense, IDA propolis, Wistar albino (Rat).