Hyaluronic acid (HA)-Gd(III) and HA-Fe(III) microgels as MRI contrast enhancing agents

ŞAHİNER N., UMUT E., Suner S., ŞAHİNER M., Culha M., Ayyala R. S.

CARBOHYDRATE POLYMERS, cilt.277, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 277
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.carbpol.2021.118873
  • Dergi Adı: CARBOHYDRATE POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Hyaluronic acid (HA), HA-Gd(III) microgel, HA-Fe(III) microgel, Blood compatible, Magnetic resonance imaging (MRI), Fe(II) chelating
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Hyaluronic acid (HA) was crosslinked with Gd(III) and Fe(III) ions rendering physically crosslinked HA-metal(III) microgels as magnetic resonance imaging (MRI) enhancing contrast agents. These HA-Gd(III) and HA-Fe(III) microgels are injectable with size range, 50-5000 nm in water. The same isoelectric point, pH 1.2 +/- 0.1, was measured for both microgels. HA-Gd(III) and HA-Fe(III) microgels are hemo-compatible biomaterials and can be safely used in intravascular applications up to 1000 mu g/mL concentration. Furthermore, no significant toxicity was attained as 95 +/- 8 and 81 +/- 2% cell viability on L929 fibroblast cells at 100 mu g/mL of HA-Gd(III) and HA-Fe (III) microgels were measured. Moreover, HA-Gd(III) microgels were found to afford significant contrast improvement capability in MRI with proton relaxivity, r1 = 2.11 mM-1 s-1, comparable with the values reported for Gd(III) labeled functionalized HA gel systems and commercial Gd based contrast agents.