Glucose monitoring by a biosensor based on mercury thin film electrodes

SEZGİNTÜRK M. K., Goktug T., DİNÇKAYA E.

ANALYTICAL LETTERS, vol.41, no.6, pp.1074-1082, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 6
  • Publication Date: 2008
  • Doi Number: 10.1080/00032710801978483
  • Journal Name: ANALYTICAL LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1074-1082
  • Keywords: biosensor, Clark electrode, glucose, glucose oxidase, mercury thin film, ANODIC-STRIPPING VOLTAMMETRY, GLASSY-CARBON ELECTRODES
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Mercury film electrodes consist of a thin film of mercury deposited on an electrode surface ( typically glassy carbon) by reduction of a mercury (II) salt in solution. The surface area/volume ratio is larger for the mercury film electrode, and this electrode is more stable than mercury drop electrode, which allows a faster stirring rate to be used in the deposition step. An enzyme electrode is described, based on glucose oxidase immobilized by gelatin and glutaraldehyde and held over a glassy carbon electrode coated with a thin mercury film. This biosensor responds fast and linearly to glucose in a wide concentration range, which is significant because monitoring of glucose levels is a critical component of diabetes care. Certain optimization and characterization studies were carried out. Average value, standard deviation (SD), and variation coefficient ( CV) were calculated with the help of the repeatability studies. Finally, glucose content of human blood samples was monitored with the help of the biosensor presented.