An amperometric inhibitor biosensor for the determination of reduced glutathione (GSH) without any derivatization in some plants

Sezginturk M. K., DINCKAYA E.

BIOSENSORS & BIOELECTRONICS, vol.19, no.8, pp.835-841, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 8
  • Publication Date: 2004
  • Doi Number: 10.1016/j.bios.2003.08.012
  • Journal Name: BIOSENSORS & BIOELECTRONICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.835-841
  • Keywords: ascorbate oxidase, glutathione determination, GSH, GSSG, inhibitor biosensors, PERFORMANCE LIQUID-CHROMATOGRAPHY, FLOW-INJECTION DETERMINATION, QUANTITATIVE-DETERMINATION, ELECTROCHEMICAL DETECTION, GAMMA-GLUTAMYLCYSTEINE, OXIDIZED GLUTATHIONE, BIOLOGICAL THIOLS, OXYGEN-TOXICITY, HUMAN PLASMA, SEPARATION
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

In this study, an amperometric biosensor based on cucumber tissue homogenate was developed for the determination of glutathione. Cucumber (Cucumis sativus L.) tissue homogenate was used as the biological material. The cucumber tissue homogenate was cross-linked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The principle of the measurements was based on the determination of the decrease in the differentiation of oxygen level which had been caused by the inhibition of ascorbate oxidase in the biological material by glutathione. Determinations were carried out by standard curves which were obtained by the measurement of the decrease in the consumed oxygen level related to glutathione concentration.