A Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors

SEZGİNTÜRK M. K., DİNÇKAYA E.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.39, no.5, pp.281-288, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.3109/10731199.2011.560119
  • Journal Name: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.281-288
  • Keywords: beta-galactosidase, glucose oxidase, biosensor, enzyme activity, electropolymerization, ALKALINE-PHOSPHATASE, ESCHERICHIA-COLI, IN-VIVO, SURFACES, ENZYME, LABEL, WATER
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

beta-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor beta-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. beta-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for beta-galactosidase detection was in the range of 0.0118 U mL(-1) to 0.47 U mL(-1) and a shorter response time of similar to 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of beta-galactosidase activity.