Quantitative Analysis of a Promising Cancer Biomarker, Calretinin, by a Biosensing System Based on Simple and Effective Immobilization Process

Asav E., Sağıroğlu A., Sezginturk M. K.

ELECTROANALYSIS, vol.28, no.2, pp.334-342, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 2
  • Publication Date: 2016
  • Doi Number: 10.1002/elan.201500324
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.334-342
  • Keywords: Calretinin, epichlorohydrin, cancer biomarker, immunosensor, Electrochemical Impedance Spectroscopy, SELF-ASSEMBLED MONOLAYERS, SURFACE-PLASMON RESONANCE, HYDROXYL-GROUPS, PROTEIN, GOLD, EXPRESSION, ACTIVATION, IMMUNOHISTOCHEMISTRY, ADSORPTION, DIAGNOSIS
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Calretinin (CAL) is calcium binding protein, and its levels in blood and cerebrospinal fluids are increased, since its expression is increased various cancer types. A novel biosensor system fabricated by immobilization of a specific antibody to CAL, anti-Calretinin (anti-CAL), onto a gold electrode surface via an effective covalent binding method using mercaptohexanol, epichlorohydrin, and ethanolamine was reported for the sensitive, selective, and accurate analysis of CAL. The proposed biosensor showed a linear calibration range between 1 ng/mL and 5 ng/mL. LOD and LOQ values were determined as 0.11 ng/mL and 0.38 ng/mL, respectively. The standard deviation related to the reproducibility of the new biosensor system was calculated as 3.95%. Lastly, in order to state the applicability of the biosensor to early diagnosis of CAL in practice, artificial serum samples spiked with CAL have been analyzed by the proposed biosensor.