Pseudomonas putida Based Amperometric Biosensors for 2,4-D Detection

ODACI DEMİRKOL D., Sezgintuerk M. K., TİMUR S., Pazarliolu N., Pilloton R., DİNÇKAYA E., ...More

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, vol.39, no.1, pp.11-19, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1080/10826060802589460
  • Journal Name: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.11-19
  • Keywords: 2,4-D, Herbicides, Microbial biosensors, Pseudomonas putida, Screen printed electrodes, CHROMATOGRAPHY-MASS-SPECTROMETRY, GAS-CHROMATOGRAPHY, ORGANOPHOSPHORUS PESTICIDES, ACID HERBICIDES, DERIVATIZATION, RESIDUES
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investigated. Substrate specificities for various phenolic compounds were also searched. In repeatability studies, variation coefficients and standard deviations for both type of systems were calculated; SPG and Clark electrodes were calculated and results are given as a comparison of two systems. Finally, the biosensors were applied to 2,4-D determination in a real herbicide sample.