Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3

Canbaz M. C., Simsek C. S., Sezginturk M. K.

ANALYTICA CHIMICA ACTA, vol.814, pp.31-38, 2014 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 814
  • Publication Date: 2014
  • Doi Number: 10.1016/j.aca.2014.01.041
  • Journal Name: ANALYTICA CHIMICA ACTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.31-38
  • Keywords: Cancer biomarkers, HER-3, Anti-HER-3, Single frequency impedance, Biosensor, GROWTH-FACTOR RECEPTOR, BREAST-CANCER, EXPRESSION, DISPERSION, ANTIBODY, THERAPY, FAMILY
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.