One step synthesis of an amino acid derived particles, poly(L-Arginine) and its biomedical application

ŞAHİNER N.

POLYMERS FOR ADVANCED TECHNOLOGIES, cilt.33, sa.3, ss.831-842, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/pat.5559
  • Dergi Adı: POLYMERS FOR ADVANCED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Applied Science & Technology Source, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.831-842
  • Anahtar Kelimeler: antimicrobial, antioxidant, biomedical application, L-Arginine, poly(amino acid), poly(L-Arginine) particles, POLY-L-ARGININE, ANTIOXIDANT ACTIVITY, OXIDATIVE STRESS, DRUG-DELIVERY, NITRIC-OXIDE, PROTEIN, DENDRIMER, NANOCARRIERS, RIBOFLAVIN, METABOLISM
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Herein, single step synthesis of poly(L-Arginine) (p(L-Arg)) particles was reported for the first time via microemulsion crosslinking method using tetrakis(hydroxymethyl) phosphonium chloride (THPC) as the crosslinking agent. The C-P vibrational FT-IR peaks of the p(L-Arg) particles observed at 1035 cm(-1) as well as elemental analysis results corroborated the successful crosslinking of native L-Arg molecules with THCP. Spherical-shaped p(L-Arg) particles visualized by SEM analysis ranged 1-10 mu m in size in dry state. The important parameters of p(L-Arg) particles affecting their biomedical potential use such as degradability, blood compatibility, antioxidant and antimicrobial properties were thoroughly investigated. Accordingly, 49.2 +/- 6.7%, 68.1 +/- 4.8%, and 62.5 +/- 7.1% of the p(L-Arg) particles were degraded at pH 5.4, 7.4, and 9.0, respectively, in about 200 h. The p(L-Arg) particles exhibited fascinating blood compatibility with less than 4% hemolysis induction and more than 95% blood clotting index at 2 mg/mL concentration. The antioxidant activity of p(L-Arg) particles was measured as 0.66 +/- 0.06 mu M Trolox equivalent g(-1) by means of TEAC assay. Moreover, the antimicrobial activity of L-Arg amino acid against Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 10145, gram-positive Staphylococcus aureus ATCC 6538, B. subtilis ATCC 6633 bacteria, and Candida albicans ATCC 10231 yeast strains was significantly enhanced in the form of p(L-Arg) particles. Furthermore, drug loading and release performances of p(L-Arg) particles were also investigated using naproxen and riboflavin as active pharmaceuticals. Based on drug release studies performed in PBS at pH 7.4, 73.9 +/- 12.6% of loaded naproxen was released in 90 min, whereas 62.1 +/- 4.6% of loaded riboflavin was released in 135 min.