Research Information System
ACTA SOCIETATIS BOTANICORUM POLONIAE, vol.92, no.1, pp.1-11, 2023 (SCI-Expanded)
Verbascum scamandri Murb. known as “Kazdağı Mullein” is an endemic species in
Türkiye and is classified as an endangered (EN) species. e aim of this study is
to establish an efficient callus culture for V. scamandri and analyze the amounts of
verbascoside, luteolin, and aucubin metabolites of calli samples. Leaf explants were
cultured on MS medium with cytokinin (BAP, Kin, 0, 0.5, 1, 2, 3 mg/L) and auxin
(NAA, 2,4-D, 0, 0.1, 0.5, 1 mg/L), 1 g/L PVP, 3% sucrose, and 0.7% agar for callus
induction. Callus tissue in MS with 2 mg/L Kin, 0.5 mg/L Kin + 0.5 mg/L 2,4-D,
2 mg/L Kin + 0.5 mg/L 2,4-D, and 3 mg/L Kin + 0.5 mg/L 2,4-D was proliferated in
MS basal medium containing PGR at the same concentrations and combinations
as the callus induction media. Verbascoside, luteolin, and aucubin were quantified
in leaf samples of the in vivo collected plants, leaf samples of in vitro growing plants,
and calli using HPLC-DAD. According to the results, the verbascoside content in
the leaf of collected plants was 7.03 mg/g, luteolin was 0.66 mg/g, and aucubin
was 2.99 mg/g. e leaf of in vitro plants had 1.62 mg/g verbascoside, 0.18 mg/g
luteolin, and 1.32 mg/g aucubin. Whereas, the maximum content of secondary
metabolites in the callus samples was observed 13.77 mg/g verbascoside in MS
medium with 2 mg/L Kin, 0.51 mg/g luteolin in MS medium with 2 mg/L Kin +
0.5 mg/L 2,4-D, and 9.32 mg/g aucubin in 0.5 mg/L Kin + 0.5 mg/L 2,4-D.