Potential Applications of Endo-beta-N-Acetylglucosaminidases From Bifidobacterium longum Subspecies infantis in Designing Value-Added, Next-Generation Infant Formulas

Duman H., KAPLAN M., Arslan A., ŞAHUTOĞLU A. S., Kayili H. M., Frese S. A., ...Daha Fazla

FRONTIERS IN NUTRITION, cilt.8, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 8
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3389/fnut.2021.646275
  • Dergi Adı: FRONTIERS IN NUTRITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Directory of Open Access Journals
  • Anahtar Kelimeler: human milk oligosaccharides, N-glycans, endo-&#946, -N-acetylglucosaminidase, bifidobacteria, infant formula, HUMAN-MILK OLIGOSACCHARIDES, CARBON LIQUID-CHROMATOGRAPHY, BREAST-FED INFANTS, CRYSTAL-STRUCTURE, CALCIUM-ABSORPTION, MICROFLUIDIC CHIP, ESCHERICHIA-COLI, FEEDING INFANTS, GUT MICROBIOTA, IN-VITRO
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Human milk is the optimal source of infant nutrition. Among many other health benefits, human milk can stimulate the development of a Bifidobacterium-rich microbiome through human milk oligosaccharides (HMOs). In recent years, the development of novel formulas has placed particular focus on incorporating some of the beneficial functional properties of human milk. These include adding specific glycans aimed to selectively stimulate the growth of Bifidobacterium. However, the bifidogenicity of human milk remains unparalleled. Dietary N-glycans are carbohydrate structures conjugated to a wide variety of glycoproteins. These glycans have a remarkable structural similarity to HMOs and, when released, show a strong bifidogenic effect. This review discusses the biocatalytic potential of the endo-beta-N-acetylglucosaminidase enzyme (EndoBI-1) from Bifidobacterium longum subspecies infantis (B. infantis), in releasing N-glycans inherently present in infant formula as means to increase the bifidogenicity of infant formula. Finally, the potential implications for protein deglycosylation with EndoBI-1 in the development of value added, next-generation formulas are discussed from a technical perspective.