Determination of C-reactive protein by PAMAM decorated ITO based disposable biosensing system: A new immunosensor design from an old molecule


KARABOĞA M. N. S., SEZGİNTÜRK M. K.

TALANTA, vol.186, pp.162-168, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 186
  • Publication Date: 2018
  • Doi Number: 10.1016/j.talanta.2018.04.051
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.162-168
  • Keywords: 11-cyarioundecyltrimethoxysilane, C-reactive protein (CRP), Biosensor, Electrochemical impedance spectroscopy (EIS), PAMAM dendrimers, LABEL-FREE DETECTION, COGNITIVE FUNCTION, CRP DETECTION, SERUM, IMMOBILIZATION, ASSOCIATION, DIAGNOSIS, DISEASE, LEPTIN
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

This paper demonstrates a new and sensitive electrochemical immunosensor for the analysis of C-reactive protein, an important marker of inflammation. Indium Tin Oxide (ITO) disposable sheets were modified by using 11-cyanoundecyltrimethoxysilane (CUTMS) and PAMAM dendrimers (G:1 amino surfaces) for the first time to immobilize the anti-CRP antibody via covalent interactions. Cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), as well as square wave voltammetry (SWV) methods, were applied to characterize the immobilization stages of anti-CRP and to determine the CRP concentrations. Charge transfer resistance changes were highly linear and sensitive to CRP concentration in the range 21-6148 fg mL(-1) and were associated with a limit of detection of 0.34 fg mL(-1). The system had acceptable repeatability (6A5%, n = 18) and good storage stability (4.5% loss after 6 weeks). Moreover, the binding characterization of CRP to anti-CRP was monitored by a single frequency impedance technique. The amount of CRP in human serum samples was analyzed with a fabricated biosensor to determine the feasibility of the biosensing system for medical purposes. We suggest that 11-CUTMS, a new silanization agent, is ideal for biosensor applications.