Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression

Özbilen A., Taşkın K. M.

International Journal of Innovative Approaches in Agricultural Research, vol.6, no.2, pp.112-128, 2022 (Peer-Reviewed Journal)


Genomic imprinting is parent-of-origin specific gene expression in embryo nourishing tissues endosperm and placenta in flowering
plants and mammals, respectively. Seeds are formed with double fertilization in flowering plants and the endosperm has a 3n

me set with the contribution of 2 maternal and 1 paternal genome. Any deviation from this ratio (2m+1p) results in seed
abortion in many species, however, apomict species modify their gametogenesis or fertilization to survive.
Boechera divaricarpa is
a dip
loid apomict plant species that can produce seeds with a 4m:1p parental genome ratio in endosperm and produce viable
SUVH7, on the other hand, is a histone methyltransferase that has a catalytic SET domain responsible for epigenetic control
of gene
expression. In this study, we characterized the structures of the SUVH7 gene and compared the mRNA levels of SUVH7 in
diploid apomict and sexual
Boechera spp. in unopened immature buds and manually pollinated siliques representing the -pre and
post pollination stages, respectively. The expression level of SUVH7 in apomict B. divaricarpa has reached the max level 48 hours
later following pollination, while in sexual
B. stricta its expression level has dramatically decreased. Therefore, our study suggests
e importance of epigenetic reprogramming in apomicts during seed development since chromatin marks via SUVH7 are
commonly associated with the activation of transcription in plants