CTLA4+rs231775 gene polymorphism increases PCOS, regardless of the levels of interleukin-6 and tumor necrosis factor-α in the serum

Beyazıt F., Çiçekliyurt M. M., Turkon H., Ünsal M. A., Pek İ. E.

ANNALS OF CLINICAL AND ANALYTICAL MEDICINE, cilt.8, sa.14, ss.696-701, 2023 (ESCI)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 14
  • Basım Tarihi: 2023
  • Doi Numarası: 10.4328/acam.2163
  • Dergi Adı: ANNALS OF CLINICAL AND ANALYTICAL MEDICINE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.696-701
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Aim: Polycystic ovarian syndrome (PCOS) is a long-standing inflammation-related disease with increased levels of circulating pro-inflammatory markers. By

affecting inflammatory cytokine production, cytotoxic T lymphocyte-associated antigen (CTLA-4) polymorphism can alter the immune system and trigger

distinct disease states. The aim of the study was to investigate if CTLA4 polymorphism is associated with PCOS, and if so, (2) whether this situation influences

serum interleukin-6 (IL-6) and TNF-alpha in PCOS.

Material and Methods: CTLA4+rs231775 gene polymorphism with IL-6 and TNF-α levels were determined in 92 PCOS women and 88 healthy controls. Study

groups were further subdivided according to body mass index (BMI) and the degree of insulin resistance (IR), and comparisons were made within each study

group.

Results: The prevalence of the A allele of single nucleotide polymorphism (SNP) rs231775 was more frequent in PCOS women compared with healthy controls

[OR: 1.99, 95% CI:1.273-3.107, p =0.0023]. The heterozygous genotype was also shown to be strongly associated with PCOS development [OR: 3.041,

95%CI:1.604-5.766, p=0.0005]. Although TNF-α levels of PCOS patients were detected to be elevated, no difference was found in the study groups with

respect to serum IL-6 levels. In addition, no association was observed between CTLA4+rs231775 polymorphism and serum pro-inflammatory cytokine levels.

Discussion: The present study demonstrates for the first time that CTLA4+rs231775 gene polymorphism increases susceptibility to PCOS 2 times more in the

case of A allele carriage and 3 times more in heterozygous individuals, independent from the long-standing low-grade inflammatory disease state encountered

in patients with PCOS.