Research Information System
10th International Drug Chemistry Conference, Antalya, Turkey, 10 - 13 March 2022, pp.39
We understood along with the Covid-19
pandemic of the last few years, has become more clear that the most important
problem is health for humanity. With the increase in cancer cases in recent
years the increased in research on the treatment of cancer diseases. The
intensive use of herbal preparations in researches caused medicinal plants to
the foreground. The use of the wild verbena in traditional folk medicine in the
treatment of some diseases and some anticancer studies have been found in the
literature checks. The wild verbena was chosen as the main material of this
study because it is a promising plant in cancer treatment. Wild verbena (Verbena
officinalis L.) flowered plants were harvested in the Çanakkale city at
July. Dried and milled wild verbena powder were subjected to ethanol
extraction. Phenolic components of wild verbena ethanol extract by LC-MS/MS
analysis were determined as caffeic acid, verbascoside, syringic acid,
salicylic acid, protocatechuic acid, 2,5-Dihydroxybenzoic acid, abscisic acid,
gallic acid, apigenin, luteolin, trans ferulic acid, catechin, jasmonic acid,
and quercetin. Caffeic acid, verbascoside, syringic acid, salicyclic acid, and
protocatechuic acid were the major components. The cytotoxic effects of the
plant extract diluted in different rates on the cancer cell lines and healthy
liver cell lines were investigated in different periods for 24 hours and 48
hours in vitro conditions. No cytotoxic effect of 6.25% diluted plant extract
was observed in 24 and 48 hours on the healthy liver cell lines. The 6.25%
diluted wild verbena ethanol extract causes 29% death in the cancer cell lines
in a 24-hour period, while it causes 53% death in the cancer cell lines in a
48-hour period. According to these results, wild verbena ethanol extract with
dilution in 6.25% shows promise in cancer treatment.