Methanol, acetone and ethyl acetate extracts obtained from Verbascum pinnatifidum Vahl. and V. antinori Boiss. et Heldr were investigated for their bioactive composition, antimicrobial and antibiofilm activity. Antimicrobial and antibiofilm activity was determined with Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Staphylococcus haemolyticus ATCC 43252 and Candida albicans ATCC 10231 by the disk diffusion, minimum inhibitory concentration and minimum bactericidal or fungicidal concentration and microplate biofilm method, respectively. Bioactive compounds analyses reveal the presence of coumarins, cardiac glycosides, quinones, flavanones. It was revealed that extracts of V. antinori has more antimicrobial activity than V. pinnatifidum extracts against test microorganisms. The lowest MIC value was recorded by V. pinnatifidum methanol extract against A. baumanii ATCC 19606 (0.3125 +/- 0.01). Antibiofilm activities of three extracts of V. pinnatifidum and V. antinori have been showed an inhibition percentage range of 8.93 - 92.18% and 14.56 - 91.19%, respectively.