Investigation of Synthesis, Characterization, and Biological Activities of Water-Soluble Polymer with Horseradish Peroxidase Enzyme


Dilek Şenol Bahçeci S., Neslihan Demir D.

Polymer Science - Series B, vol.64, no.5, pp.733-748, 2022 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.1134/s1560090422700488
  • Journal Name: Polymer Science - Series B
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, Compendex, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.733-748
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

© 2022, Pleiades Publishing, Ltd.Abstract: 7-Amino-4-hydroxy-2-naphthalene sulfonic acid (AHNSA) was converted into p(AHNSA-HRP) by enzyme-catalyzed oxidative polymerization in the presence of horseradish peroxidase enzyme using hydrogen peroxide in dioxane solvent. The structural analysis of AHNSA and its enzyme-catalyzed oxidative polymer, were taken by 1H NMR, 13C NMR, FTIR measurements. The optical features by UV–Vis spectrophotometry, the decomposition temperatures by TGA and the morphological properties by SEM were determined. For biological activities of AHNSA and synthesized enzymatic polymer, DNA cleavage and DNA binding activities were done by agarose gel electrophoresis and by UV–Vis spectrophotometer, respectively. It was observed that the polymer interacted with DNA electrostatically in its DNA binding activity. As a result of the agarose gel electrophoresis studies, it was seen that the polymer cleaved the DNA both hydrolytically and oxidatively. Antioxidant properties, 2,2-diphenyl-1-picryl-hydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power detection were studied by three different methods. Antimicrobial activities were investigated against various bacterial and yeast cultures using the minimum inhibition concentration method. It was observed that both antioxidant and antimicrobial properties of the new enzyme-catalyzed oxidative polymer were higher than that of AHNSA.