Direct shoot organogenesis of Digitalis trojana Ivan., an endemic medicinal herb of Turkey


ÇÖRDÜK N. , Aki C.

AFRICAN JOURNAL OF BIOTECHNOLOGY, cilt.9, ss.1587-1591, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 9 Konu: 11
  • Basım Tarihi: 2010
  • Doi Numarası: 10.5897/ajb10.1822
  • Dergi Adı: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Sayfa Sayıları: ss.1587-1591

Özet

An efficient protocol for in vitro propagation of Digitalis trojana Ivan. was developed via adventitious shoot regeneration. Leaf explants were cultured on MS which were supplemented with different concentrations of NAA (0.1, 0.5, 1.0 mg/ml) and BAP (0.1, 0.5, 1.0, 3.0, 5.0 mg/ml) for shoot formation. Adventitious shoots were formed on leaf explants within three weeks in culture. The best shoot proliferation was observed among explants cultured on MS medium with 0.1 mg/ml NAA + 3.0 mg/ml BAP. Regenerated shoots were multiplicated by subculture. Then they were cultured on MS with 0.1% (w/v) activated charcoal for root formation. All of the in vitro regenerated plantlets were successfully acclimatized ex vitro and then grown healthy.