Direct shoot organogenesis of Digitalis trojana Ivan., an endemic medicinal herb of Turkey


Çördük N., Aki C.

AFRICAN JOURNAL OF BIOTECHNOLOGY, vol.9, no.11, pp.1587-1591, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 11
  • Publication Date: 2010
  • Doi Number: 10.5897/ajb10.1822
  • Journal Name: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1587-1591
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

An efficient protocol for in vitro propagation of Digitalis trojana Ivan. was developed via adventitious shoot regeneration. Leaf explants were cultured on MS which were supplemented with different concentrations of NAA (0.1, 0.5, 1.0 mg/ml) and BAP (0.1, 0.5, 1.0, 3.0, 5.0 mg/ml) for shoot formation. Adventitious shoots were formed on leaf explants within three weeks in culture. The best shoot proliferation was observed among explants cultured on MS medium with 0.1 mg/ml NAA + 3.0 mg/ml BAP. Regenerated shoots were multiplicated by subculture. Then they were cultured on MS with 0.1% (w/v) activated charcoal for root formation. All of the in vitro regenerated plantlets were successfully acclimatized ex vitro and then grown healthy.