Combining the bioimprinting technique with lipase immobilization for interesterification
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.18, no.7, pp.621-625, 2002 (SCI-Expanded)
- Publication Type: Article / Article
- Volume: 18 Issue: 7
- Publication Date: 2002
- Doi Number: 10.1023/a:1016855931559
- Journal Name: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.621-625
- Çanakkale Onsekiz Mart University Affiliated: Yes
Abstract
Lipases were bioimprinted, immobilized and bioimprinted-immobilized (coupled technique), and interesterification activities were compared. Bioimprinted-immobilized enzyme preparations gave 4-6.2-fold activity enhancements compared to solid enzyme preparations supplied by manufacturers. This increase was higher than that of bioimprinting alone and immobilization alone. Solvent-free media were equally effective and water addition caused erasure of bioimprinting. Also, new preparations showed higher thermostability and reusability.