Combining the bioimprinting technique with lipase immobilization for interesterification

Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.18, no.7, pp.621-625, 2002 (SCI-Expanded) identifier identifier


Lipases were bioimprinted, immobilized and bioimprinted-immobilized (coupled technique), and interesterification activities were compared. Bioimprinted-immobilized enzyme preparations gave 4-6.2-fold activity enhancements compared to solid enzyme preparations supplied by manufacturers. This increase was higher than that of bioimprinting alone and immobilization alone. Solvent-free media were equally effective and water addition caused erasure of bioimprinting. Also, new preparations showed higher thermostability and reusability.