Combining the bioimprinting technique with lipase immobilization for interesterification


Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, cilt.18, ss.621-625, 2002 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 18 Konu: 7
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1023/a:1016855931559
  • Dergi Adı: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Sayfa Sayıları: ss.621-625

Özet

Lipases were bioimprinted, immobilized and bioimprinted-immobilized (coupled technique), and interesterification activities were compared. Bioimprinted-immobilized enzyme preparations gave 4-6.2-fold activity enhancements compared to solid enzyme preparations supplied by manufacturers. This increase was higher than that of bioimprinting alone and immobilization alone. Solvent-free media were equally effective and water addition caused erasure of bioimprinting. Also, new preparations showed higher thermostability and reusability.