Combining the bioimprinting technique with lipase immobilization for interesterification


Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.18, no.7, pp.621-625, 2002 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 7
  • Publication Date: 2002
  • Doi Number: 10.1023/a:1016855931559
  • Title of Journal : WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Page Numbers: pp.621-625

Abstract

Lipases were bioimprinted, immobilized and bioimprinted-immobilized (coupled technique), and interesterification activities were compared. Bioimprinted-immobilized enzyme preparations gave 4-6.2-fold activity enhancements compared to solid enzyme preparations supplied by manufacturers. This increase was higher than that of bioimprinting alone and immobilization alone. Solvent-free media were equally effective and water addition caused erasure of bioimprinting. Also, new preparations showed higher thermostability and reusability.