Two biosensors for phenolic compounds based on mushroom (Agaricus bisporus) homogenate: Comparison in terms of some important parameters of the biosensors

Teke M., Sezgintuerk M. K., DİNÇKAYA E., Telefoncu A.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.38, sa.1, ss.51-60, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 1
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/10826060701774346
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.51-60
  • Anahtar Kelimeler: enzyme based biosensors, polyphenol oxidase, biosensor stability, bioimprinted biosensors, GLUCOSE-OXIDASE, CROSS-LINKING
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other.