In this study, the electrochemical behavior of eugenol, a widely used herbal drug, was investigated at a pencil graphite electrode (PGE). A low-cost, disposable, sensitive and selective electrochemical sensor is proposed for the determination of eugenol by recording its differential pulse voltammograms in Britton-Robinson buffer solution containing 0.1 M KCl with pH of 2.0 at the PGE. The PGE displayed a very good electrochemical behavior with significant enhancement of the peak current compared to a glassy carbon electrode. Under experimental conditions, the PGE had a linear response range from 0.3 mu M to 50.0 mu M eugenol with a detection limit of 0.085 mu M (based on 3S(b)). Relative standard deviations of 2.4 and 4.8% were obtained for five successive determinations of 30.0 and 5.0 mu M eugenol, respectively, which indicate acceptable repeatability. This voltammetric method was successfully applied for the direct determination of eugenol in real samples. The effect of various interfering compounds on the eugenol peak current was also studied. (C) 2015 Elsevier B.V. All rights reserved.