THERAPEUTIC POTENTIAL OF OZONE AND L-CARNITINE COMBINED ADMINISTRATIONS AGAINST EXPERIMENTALLY-INDUCED ACETAMINOPHEN


Creative Commons License

Paldır E., Eroğlu H. A.

Journal Of Animal And Plant Sciences, vol.31, pp.981-987, 2021 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 31
  • Publication Date: 2021
  • Doi Number: 10.36899/japs.2021.5.0331
  • Title of Journal : Journal Of Animal And Plant Sciences
  • Page Numbers: pp.981-987

Abstract

Acetaminophen is a widely used medicine with antipyretic and analgesic effects. N-acetyl-P-benzoquinoneimine accumulation after a high-dose of acetaminophen leads to hepatotoxicity, depletion of glutathione stores, and suppression of the antioxidant defense mechanism. As a result, N-acetyl-P-benzoquinoneimine cannot be eliminated from the body and hepatotoxicity occurs. In this study, the effects of the separate and combined use of ozone and L-carnitine in high- dose acetaminophen induced hepatotoxicity was investigated. A total of 56 female Wistar albino rats were randomly divided into 8 groups of seven rats. Acetaminophen was administered orally as a single dose to induce liver damage, and 0.5 mg/kg of 95% oxygen plus 5% ozone gas mixture was administered intraperitoneally. After 1-hour APAP administration, L-carnitine were given 500 mg/kg by intraperitoneally. Serum and tissue oxidant/antioxidant parameters were measured to deduce their combined effect. ANOVA and Tukey’s multiple comparison test were used for statistical analysis. Acetaminophen+L-carnitine+ozone administration caused a significant decrease in the levels of serum malondialdehyde, total oxidant level, aspartate aminotransferase, alanine aminotransferase, and tissue malondialdehyde, total oxidant status levels, while it led to a significant increase in the levels of tissue and serum total antioxidant levels. Nonetheless, a clear evidence of superiority over the sole use of ozone or L-carnitine in acetaminophen induced hepatotoxicity was not present. It was concluded that ozone, L-carnitine and ozone+L-carnitine treatments in acetaminophen induced hepatotoxicity protected the organism against the harmful effects of free radicals and activated the antioxidant mechanism by suppressing oxidative stress.