Acetaminophen is a widely used medicine with antipyretic and analgesic effects. N-acetyl-P-benzoquinoneimine
accumulation after a high-dose of acetaminophen leads to hepatotoxicity, depletion of glutathione stores, and suppression
of the antioxidant defense mechanism. As a result, N-acetyl-P-benzoquinoneimine cannot be eliminated from the body
and hepatotoxicity occurs. In this study, the effects of the separate and combined use of ozone and L-carnitine in high- dose acetaminophen induced hepatotoxicity was investigated. A total of 56 female Wistar albino rats were randomly
divided into 8 groups of seven rats. Acetaminophen was administered orally as a single dose to induce liver damage, and
0.5 mg/kg of 95% oxygen plus 5% ozone gas mixture was administered intraperitoneally. After 1-hour APAP
administration, L-carnitine were given 500 mg/kg by intraperitoneally. Serum and tissue oxidant/antioxidant parameters
were measured to deduce their combined effect. ANOVA and Tukey’s multiple comparison test were used for statistical
analysis. Acetaminophen+L-carnitine+ozone administration caused a significant decrease in the levels of serum
malondialdehyde, total oxidant level, aspartate aminotransferase, alanine aminotransferase, and tissue malondialdehyde,
total oxidant status levels, while it led to a significant increase in the levels of tissue and serum total antioxidant levels.
Nonetheless, a clear evidence of superiority over the sole use of ozone or L-carnitine in acetaminophen induced
hepatotoxicity was not present. It was concluded that ozone, L-carnitine and ozone+L-carnitine treatments in
acetaminophen induced hepatotoxicity protected the organism against the harmful effects of free radicals and activated
the antioxidant mechanism by suppressing oxidative stress.