Research Information System
Journal of Agriculture Faculty of Ege University, vol.61, no.3, pp.367-381, 2024 (Scopus)
Objective: The effect of 24-epibrassinolide (EBR) application against NaCl stress in three cotton (Gossypium hirsutum L.) cultivars (Nazilli 84-S, Carmen, White Gold 119) was investigated. Material and Methods: After the seeds were treated with 3 µM EBR solution for 24 h, they were sowed in pots and watered with Hoagland nutrient solution containing 75 and 150 mM NaCl. Plant dry weight, membrane permeability, stomatal density, relative water content, photosynthetic pigment contents, antioxidative enzyme activities, and DNA content were determined in the plants harvested at the beginning of the flowering period. Results: EBR treatments reduced the decrease in dry weights, relative water content, and stomatal density of plants. Membrane permeability increased with increasing salt concentration in all three varieties, but this increase decreased with the EBR treatment. Antioxidative enzyme activities (SOD and POD) increased significantly with NaCl application. NaCl application reduced stoma-guard cell number, photosynthetic pigment and DNA content, but this decrease was improved by EBR treatment. Conclusion: These results show a salt-dependent increase in antioxidative enzyme activity in cotton varieties and reveal the stress-relieving effects of EBR by helping to maintain water potential and membrane integrity in cotton plants under salt stress.