This study aimed to determine the different vibration frequencies (4, 8, 12, and 16 Hz) and packaging trays and films (Polypropylene trays + microperforated films (MP) and Polystyrene foam trays + polyvinyl chloride stretch films (PVC)) effects on mushroom quality. The quality of the mushrooms in MP was reduced with the increment in the frequency, whereas the effect of the frequency was only detrimental in the mushrooms packaged with PVC at 16 Hz. Oxygen concentration reached the equilibrium state at the level of 15% in PVC and 9% in MP groups. The whiteness index of mushrooms in MP was between 25 and 40, whereas it was around 20-30 in PVC films. However, changes in electrolyte leakage, pH, and hardness were minimal in the mushrooms packaged with PVC. As a result, PVC stretch films reduced the effect of transport frequencies, however, the optimal gas concentrations for mushrooms were only achieved using MP films.