Dopamine is a hormone and neurotransmitter occurring in a wide variety of animals, including both vertebrates and invertebrates. Chemically, it is a phenethylamine. Dopamine can be supplied as a medication that acts on the sympathetic nervous system, producing effects such as increased heart rate and blood pressure. However, since dopamine cannot cross the blood-brain barrier, dopamine given as a drug does not directly affect the central nervous system. To increase the amount of dopamine in the brains of patients with diseases such as Parkinson's disease and Dopa-Responsive Dystonia, L-DOPA (levodopa), which is the precursor of dopamine, can be given because it can cross the blood-brain barrier. In this study, a biosensor based on quince tissue homogenate was constructed for determination of dopamine. For the best results, some optimization studies such as the amount of quince tissue homogenate, gelatin and glutaraldehyde percentage, optimum temperature and pH were carried out. A linear range from 5M to 200M dopamine was obtained. Moreover, repeatability, and operational and storage stability of the biosensor were determined. Finally, the biosensor was applied to a real drug sample for the determination of dopamine content.