Double and Triple Gel Stocks Method in Pulsed Field Gel Electrophoresis


Senel U.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.10, ss.73-79, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 10 Konu: 9
  • Basım Tarihi: 2015
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Sayfa Sayıları: ss.73-79

Özet

Although pulsed-field gel electrophoresis (PFGE) is a very valuable and gold standard method in molecular typing of bacteria-based epidemic diseases, its main disadvantages are as follows; it is time-consuming, it enables simultaneous working on a very limited samples and it requires experienced personnel. This study aims to work on 2-3 times more samples during the single gel system protocol simultaneously with the application of double and triple gel system to current PFGE system. In order to ensure the perfection of DNA patterns observed in gels to be assessed as those in single system and to prevent the contamination of these DNA patterns; intermediate lamellas manufactured from 13x14 cm polystyrene (PS), acrylonitrile-butadiene-styrene (ABS) and acetyl cellulose were used.