Pyrimidine and their derivatives play an important role in the field of pharmacological activity. Also, pyrimidine compounds containing pyrido pyrimidine structure show significant biological and pharmaceutical activities. In this study, indeno [2',1':5,6]pyrido [2,3-d]pyrimidine derivative was synthesized by the one-pot condensation. This one-pot, three-component condensation took place under mild conditions; 1,3-Indandione (indanedione) (1), 6-amino uracil (2), 4-nitro benzaldehyde (3) in DMF/Et3N at 60 degrees C. The reaction was completed after 24 h. Also, eight different methods were tried for the reduction of aryl nitro group. For the selective reduction of aryl nitro group in a good yields was used under elemental sulfur (S8), sodium sulphide (Na2S) in THF/water at 100 degrees C conditions. All coumpounds were characterized by spectroscopic techniques.