Aim: Hepatitis C virus (HCV) infections is an important health problem all over the world because of inveteracy ratio and causing hepatic failure, cirrhosis and hepatocellular carcinoma. Our aim in this study is to research HCV RNA in the patients detected anti-HCV positivity with enzyme immunoassay (ELISA) and to analysis the association of HCV RNA in the serum of patients with alanine aminoransferase (ALT) levels and HCV genotypes. Material and Method: In anti-HCV positive patients came from various departments to our laboratory HCV RNA level and genotyping were researched with quantitive real time polimerase chain reaction (PCR) Montania 483 (Anatolia Geneworks HCV, Turkey) and anti-HCV tests were studied with ELISA (Advia Centaur XP, Germany), ALT results were analysed retrospectively (Olympus AU2700). Results: In 123 of Anti HCV positive 338 serum (% 37) were detected HCV RNA positivity. In the patients who have ALT level more than 40 (% 77.6), HCV RNA is positive. Relationship between HCVRNA positive patients and the patients who have ALT level more than 40 is meaningful. (p< 0,05). HCV genotype distribution is detected as genotype 1b % 84 (n=37) genotype 1a % 11,4 (n=5), genotype 3 % 2,3 (n=1), genotype 4 % 2.3 (n=1). Discussion: Consequently, in the patients who have hepatic infection with Anti HCV positivity HCV RNA must be researched with molecular methods and viral load must be detected. Genotype results in our study are concordant with our environment. In diagnosis, treatment and follow up of the patients with HCV infection, HCV RNA detection must evaluate totally taking into account HCV genotype and hepatic enzymes.