In the present report the effects of naphthalene acetic acid (N ibberellic acid (GA3), and epibrassinolide (EBr) on in vitro pollen germination of three Vitis vinifera L. cultivars were described. The basic media contained 1% agar and 20% sucrose. Pollen germination of cultivar :Amasya Beyazi' was not affected by, tile treatments and the germination rate varied between 6.16% (1.0 mg L-1 i NAA) and 3598% (5Q mg L-1 GA(3) Concentrationof 25 mg 1-4 GA(3) had the highest effect on increasing of The stimulating effect of GA(3) and EBr was noticeable incultivar the pollen germination of cultivar Kozak Beyazi' compared to the NAA, epibrassinolide and the control. The results showed that the response of the pollen grains were cultivar specific and substance specific. In general, NAA was the growth regulator that least enhanced the germination of grapevine pollen.