Low-affinity cAMP phosphodiesterase activity essential for NTH1 gene expression in Saccharomyces cerevisiae

Turgut Genç T., Solak M. Ş.

6. International Molecular Biology and Biotechnology, Afyonkarahisar, Turkey, 22 - 25 December 2017, vol.1, no.1, pp.10

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Afyonkarahisar
  • Country: Turkey
  • Page Numbers: pp.10
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Saccharomyces cerevisiae includes two genes, PDE1 and PDE2. PDE1 encodes low affinity phosphodiesterase that controls glucose and intracellular acidification-induced cAMP signalling and PDE2 encodes high affinity phosphodiesterase that protects the yeast cells from extracellular cAMP, controls the basal level of cAMP. The major determinant of stress resistance of the yeast S. cerevisiae is the Ras-cAMP pathway. Also it is already known that NTH1 is a multiple stress response gene. The regulation of NTH1 gene expression involves different signaling pathways including many transcriptional activators and repressors that regulate the expression of NTH1 gene at the transcriptional level. Despite the fact that regulation of this gene is controlled by a complicated pathway, the role of these activators and repressors  in this pathway are not known yet. So that in our research we analyzed the effect of low affinity phosphodiesterase, Pde1, on NTH1 gene expression. The transcription of NTH1 gene decreased 2-fold in ?pde1 yeast strains under normal growth conditions. Also NTH1 gene expression in ?pde1 mutant yeast strains decreased 3-fold than wild type strain under nitrogen starvation. Similarly heat stres also caused to decrease gene expressin 2-fold in ?pde1 mutant yeast strains than wild type strains. These results shows that the low affinity cyclic AMP phosphodiesterase activity and so active PKA necessary for NTH1 gene expression both in normal and stress conditions.