Trace metal contamination has become a worldwide problem for aquatic systems, as sediments act as a sink for trace metals. This study was conducted to assess geochemical fractions, sources and potential risks of trace metals (Cd, Cr, Cu, Ni, Pb, and Zn) in sediments of Umurbey stream (Canakkale-Turkey). Sediment samples were taken from seven different locations of Umurbey stream. Aqua regia wet digestion procedure was applied to determine total contents of trace metals and BCR sequential extraction procedure was applied to determine geochemical fractions of trace metals. Trace metal total values were ordered as Zn > Pb > Cr > Cu > Ni > Cd. Just because of topography, geology, and agricultural practices, S4, S5, S6, and S7 sampling points had greater total trace metal concentrations than the other locations. Potential mobility of trace metals in sediment samples was ordered as Cd (62.1%) > Zn (60.8%) > Pb (54.8%) > Cu (46.1%) > Cr (43.0%) > Ni (29.7%). Cd, Zn, Pb, and partially Cu were encountered mostly in mobile phases. Multivariate analyses revealed that pollution in sediment samples was originated from not only anthropogenic but also natural factors. Except for Zn, trace metal concentrations were found to be at tolerable levels of biota. When the contamination factor and risk assessment code results were assessed together, it was observed that Cd, Zn, and partially Pb were weakly adsorbed onto sediments, thus might pose risks on environment in the long run.