Akademik Veri Yönetim Sistemi
Applied Ecology and Environmental Research, cilt.22, sa.6, ss.5467-5481, 2024 (SCI-Expanded)
In this study, staining methods were used to improve the effective visualization of grapevine cane sections under a microscope using ‘ring light’. The stains applied to the sections included Aniline Blue, Safranin O, Toluidine Blue O, Bromophenol Blue, Basic Fuchsin, Methyl Green, Giemsa Stain, Carmine and Fast Green FCF. The natural color differences observed between the tissues were similar. Therefore, staining was proven to be the most useful tool to increase the contrast in grapevine cane tissues. Different stains reacted with different cell types and components and revealed their unique color properties. In addition to single use of stains, Carmine + Methyl Green, Fast Green FCF + Bromophenol Blue and Safranin O + Bromophenol Blue were utilized as double staining. Among these applications, staining with Aniline Blue, Bromophenol Blue, Fast Green FCF, Safranin O, and Toluidine Blue O showed effective results on cell and tissue levels. In addition, successful results were obtained from double stains. Double staining was recommended for examining grapevine cane sections under a microscope. ‘Ring light’ has been developed to improve the coloration and sharpness on the surface of the stained sections under the microscope and during photography. The ring light offers the opportunity to illuminate the entire sample by using white light, yellow light and both light intensities in different proportions.