Akademik Veri Yönetim Sistemi
7th European Congress of Pharmacology (EPHAR2016), İstanbul, Türkiye, 26 - 30 Haziran 2016, ss.243
Bacterial changes in intestinal flora are associated with metabolic and immunological
problems. Biofenols such as oleuropein with antimicrobial activities regulate intestinal flora
by reducing pathogenic bacteria. We aimed to investigate how intestinal flora is affected in
rats fed predominantly with oleuropein.
Twenty adult, male Wistar albino rats were randomly divided into two groups. Group O
(n=10) received olive leaf extract 20 mg/kg/day by intragastric gavage in addition to
standard rat chow and water for 30 days. Group C (n=10) were fed with standard rat chow
and water for 30 days. After 30 days, laparotomy was performed in all rats and one gram of
ceacal contents were collected. Sterile physiological saline solution of 9 mL was added to all
ceacal contents and consecutive 10-fold serial dilutions were prepared with a final
concentration of 10 -8 . The diluted samples were inoculated on Plate Count agar and Violet
Red Bile Glucose agar to determine the total number of enteric bacteria. After incubation at
37 °C for 48 hours, isolated bacterial counts from the plates were recorded as CFU/g (colony
forming unit/gram).
Total aerobic bacterial counts of Group O were significantly lower than Group C in all plates
inoculated with ceacal samples in every dilution (p<0.005) (Table 1).
Oleuropein reduced the bacterial count isolated by conventional aerobic culture methods
we used, however other bacteria that could not be isolated with this method were not
counted and this is a limitation of our study. It is known that patients in intensive care units
are prone to develop bacterial translocation (BT). Mortality rates will decrease by starting
enteral nutrition earlier in critically ill patients. Addition of oleuropein on enteral nutrition
solutions may be helpful in recovery of critically ill patients predisposing to BT by reducing
enteric bacterial count.
Keywords: enteric bacteria, intensive care, oleuropein, rat