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Karyelioğlu M., Karav S.

The Sixth International Symposium on Pharmaceutical and Biomedical Sciences, Gaziantep, Turkey, 26 - 28 May 2022, pp.47

  • Publication Type: Conference Paper / Summary Text
  • City: Gaziantep
  • Country: Turkey
  • Page Numbers: pp.47
  • Çanakkale Onsekiz Mart University Affiliated: Yes



Pitaya is a fruit of the genus Hylocereus with rich content, and functional bioactive components.

Recently, the production and consumption of pitaya have increased worldwide. It has important roles in

human health such as cancer chemoprevention, anti-inflammatory, anti-diabetic effects, and decreasing

risk of cardiovascular diseases. Pitaya is special for human gut health because of its glycan content.

Glycans can be found as a free or conjugated form in nature. They can reach the colon in undigested

form since humans do not have glycosidase enzymes to utilize glycans. They can be utilized by some

symbiotics as a carbon source in gut microbiota and, cause selective growth among some probiotics.

Bifidobacterium infantis has the capability of degradation and utilization of these glycans. It can release

conjugated N-glycans by its unique enzyme Endo-β-N-acetylglucosaminidase (EndoBI-1). Studies

about conjugated glycans are limited, thus released N-glycans from pitaya may have important prebiotic

effect on gut health.

Therefore, pitaya samples that are obtained from Thailand and Mersin were analyzed for protein content.

Protein quantity of samples measured with QUBIT 3.0 fluorometer and visualized. By using EndoBI-1

enzyme, pitaya N-glycans released and purified. Characterization of glycans will be performed by

MALDI-TOF-MS and HPLC-HILIC-FLD analysis. In vitro digestion model will be used for the

analysis of purified glycans’ prebiotic activity. These glycans utilization as a prebiotic will be tested on

several probiotics.

Consequently, we will have information about the pitaya N-glycan profile, and prebiotic activity studies

of bioactive N-glycans will form the basis outputs of this study. In this context, how various

glycoprotein-containing products are affected throughout the digestive tract and how they shape the

intestinal microflora will be revealed. These findings on pitaya fruit may have a role in advancing

research into the complex nature of fruit-drug interactions and their possible impact on the clinical

effects of drugs.

Key Words: Pitaya, N-glycan, prebiotic, in-vitro digestion, Endo-β-N-acetylglucosaminidase


This study was derived from the Graduate Thesis of Melda Karyelioğlu entitled as “Characterization of Pitaya NLinked

Glycans and In-vitro Identification of Potential Prebiotic Effect” supplied for partial fulfillment of the

Master’s Degree at Molecular Biology and Genetics Department at Institute of Graduate Studies at Çanakkale

Onsekiz Mart University. The study supported by The Scientific And Technological Research Council Of Turkey

2210-C Program and Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit Project

number: FYL-2021-3770.