Many work accidents occur every day in working life and employees get occupational diseases when they are exposed to risks related to their jobs. It is the duty of the employer to take all kinds of measures related to Occupational Health and Safety (OHS) in the workplace and to comply with these measures taken by the employer. In work accidents and occupational diseases, both employers and employees suffer financial and moral damage. Considering the economic aspects of the work (treatment, maintenance expenses, compensation, etc.), audits should be carried out in accordance with the legislation to prevent work accidents and occupational diseases. The positive effects of the audits on employers and employees are undoubtedly quite high. In this study, the effect of the Occupational Safety Inspections (OSI) on the Occupational Safety Cultures (OSC) of the technical staff working in the auto services operating in Çanakkale was investigated. The universe of the research is the authorized and private auto services that carry out auto maintenance and repair activities in the Çanakkale centrum. The sample of the study consists of a total of 105 technical staff working in maintenance and repair, auto body and paint and electrical works, of which 50 are authorized services, 55 are in private services. Face to face questionnaires were applied to randomly selected staff from the research universe. Survey data were analyzed using SPSS 22.0 statistical software. The findings of the analysis were discussed and suggestions were made. As a result of the statistical analysis and evaluations, it has been determined that there are significant relationships between the status of the OHS inspections in the enterprises and the OSC scales; management commitment, occupational safety priority, occupational safety communication, occupational safety education, employee participation and fatalism.