Orlistat-induced molecular bio-imprinting of microbial lipase
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, cilt.19, sa.2, ss.161-165, 2003 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 19 Sayı: 2
- Basım Tarihi: 2003
- Doi Numarası: 10.1023/a:1023216301165
- Dergi Adı: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.161-165
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
Some microbial lipases bio-imprinted with the reversible lipase inhibitor, Orlistat. Comparison of interesterification activities indicated that Orlistat bio-imprinting is equally effective as amphiphile imprinting and yields a 3.5-4.0-fold activity enhancement. Solvent-free incubation medium was as effective as hexane medium. Water addition into the incubation medium erased the bio-imprinting effect; hence, lid opening should be the mechanism of activation. This study provides additional proof to the hypothesis that lipase bio-imprinting is an active-site related phenomenon.