Orlistat-induced molecular bio-imprinting of microbial lipase


Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.19, no.2, pp.161-165, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1023/a:1023216301165
  • Title of Journal : WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Page Numbers: pp.161-165

Abstract

Some microbial lipases bio-imprinted with the reversible lipase inhibitor, Orlistat. Comparison of interesterification activities indicated that Orlistat bio-imprinting is equally effective as amphiphile imprinting and yields a 3.5-4.0-fold activity enhancement. Solvent-free incubation medium was as effective as hexane medium. Water addition into the incubation medium erased the bio-imprinting effect; hence, lid opening should be the mechanism of activation. This study provides additional proof to the hypothesis that lipase bio-imprinting is an active-site related phenomenon.