An Enzymatic Glucose Biosensor Based on a Pencil Graphite Electrode Modified with Naphthalenedimide/3,4-Ethylenedioxythiophene Conjugated Polymer and Enriched with Au Nanoparticles


CHEMISTRYSELECT, vol.7, no.2, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 2
  • Publication Date: 2022
  • Doi Number: 10.1002/slct.202103437
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Keywords: glucose biosensor, pencil graphite electrode graphite, electropolymerization, naphthalenedimide-3,4-Ethylenedioxythiophene, gold nanoparticles, OXIDASE, MATRIX, FILM
  • Çanakkale Onsekiz Mart University Affiliated: Yes


N,N '-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide (ENDI) monomer was electropolymerized on pencil graphite electrode (PGE) and used as a biosensor for glucose immobilization for the first time. After electropolymerization of ENDI monomer on PGE (AuNPs/PENDI/PGE), the intensity of the current signal was improved by using gold nanoparticles (AuNPs). The immobilization of the glucose oxidase (GOx) enzyme was carried out by using the cross-linking reagent glutaraldehyde (GA) due to the strong pi-pi* interaction of the poly- N,N '-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide (PENDI) polymer. GOx/AuNPs/PENDI/PGE designed biosensor, was characterized by a wide linear glucose determination range between 0.0009-0.33 mM with a limit of detection of 0.0407 mM and a sensitivity of 0.172 mu A/mMcm(2). Herein we have shown that GOx/AuNPs/PENDI/PGE biosensor effectively provides the enzyme immobilization and very effective for glucose determination in commercial samples.