CHEMISTRYSELECT, vol.7, no.2, 2022 (SCI-Expanded)
N,N '-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide (ENDI) monomer was electropolymerized on pencil graphite electrode (PGE) and used as a biosensor for glucose immobilization for the first time. After electropolymerization of ENDI monomer on PGE (AuNPs/PENDI/PGE), the intensity of the current signal was improved by using gold nanoparticles (AuNPs). The immobilization of the glucose oxidase (GOx) enzyme was carried out by using the cross-linking reagent glutaraldehyde (GA) due to the strong pi-pi* interaction of the poly- N,N '-bis(2-hexyl)-2,6-(3,4 ethylenedioxythiophene)-1,4,5,8-naphthalenimide (PENDI) polymer. GOx/AuNPs/PENDI/PGE designed biosensor, was characterized by a wide linear glucose determination range between 0.0009-0.33 mM with a limit of detection of 0.0407 mM and a sensitivity of 0.172 mu A/mMcm(2). Herein we have shown that GOx/AuNPs/PENDI/PGE biosensor effectively provides the enzyme immobilization and very effective for glucose determination in commercial samples.