The application of chemical fertilizer and different organic substances into the soil can increase plant yield and improve some physical and chemical properties of soil. A field experiment was conducted for 2 years to determine the effect of the application of chemical fertilizer (ammonium sulphate-21% N), control and different organic substances (farmyard manure, chicken manure, leonardite, biofertilizer,  pea + annual ryegrass, mixtures of common vetch + annual ryegrass) on aggregate stability, bulk density, pH, electrical conductivity, organic matter, total N, available  P, K, Ca, and Mg concentration of soil at a depth of 0-5 cm and 5-20 cm in annual ryegrass cultivation. The field experiment was established in a randomized complete block design with 3 replications. Some physico chemical analyzes were carried out on the soil samples taken from the study area. Farmyard manure affected the available P and K in the soil at a depth of 0-5 cm of the applications of different organic and inorganic substances. With the application of farmyard manure, the available P and K content of the soil has increased. The  application of chemical fertilizer and organic substances did not affect the remaining properties of soils taken from 2 depths. It is suggested that the long term field experiments should be conducted to determine the effect of the application of chemical fertilizer and organic substances on the properties of soil.