Sustainable Chemistry and Pharmacy, cilt.51, 2026 (SCI-Expanded, Scopus)
In this study, to the best of our knowledge, alginate was extracted from seaweeds and its direct use in packaging film formation was investigated. Alginate was extracted by optimizing the extraction yield, temperature, reaction duration, and alkali concentration. Subsequently, halloysite nanotubes (HNT) were added to enhance the mechanical strength of the resulting Alginate-based films were developed by incorporating halloysite nanotubes (HNT) to enhance mechanical stability, while thyme oil (THY) was added to improve antibacterial activity and moisture resistance (evaluated via vapor swelling behavior). The optimization of alginate extraction was performed using Response Surface Methodology (RSM) with a Central Composite Design (CCD). The optimization parameters for alginate extraction are: temperature (70, 80, 90 °C), extraction time (2, 3, 4 h) and alkali concentration (2%, 3%, 4% wt. sodium carbonate, SC). Under the optimal factors of 90 °C, 4% SC, and a duration of 4 h, the alginate yield was obtained at 21%. The findings indicated that extracted alginate from seaweeds, the resulting polysaccharide was capable of forming a film, and the incorporation of HNT significantly enhanced the mechanical strength. Furthermore, the THY additive enhanced both the moisture resistance and the antibacterial efficiency of the film against Gram-negative bacteria (Escherichia coli).