Akademik Veri Yönetim Sistemi
8th International Symposium on Pharmaceutical Sciences ISOPS-8 Proceedings and Abstracts, Ankara, Türkiye, 13 Haziran 2006, (Özet Bildiri)
In this study, a new micellar electrokinetic capillary chromatography method was developed to analyze pharmaceutical preparations containing ternary combination of paracetamol (PAR), caffeine (KAF) and propyphenazone (PRO), simultaneously by capillary electrophoresis. Best results were obtained by the use of 20 mM pH 9.0 borate buffer containing 30 mM sodium dodecylsulphate as the background electrolyte. Diflunisal (DiF) was used as internal standard. The separation was performed through a fused silica capillary (50 µm internal diameter, 44 cm total length, 35.5 cm effective length) at 25°C with the application of 3 seconds of hydrodynamic injection at 50 mbar pressure and a potential of 29 kV. Detection wavelength was 200 nm. Under these conditions, the migration times were found to be 5.17 min for PAR, 5.51 min for KAF, 7.20 min for DIF, and 9.37 min for PRO. Linearity ranges for the method were determined as 2-200 µg mL⁻¹ for PAR and KAF and 3-200 µg mL⁻¹ for PRO. Limit of detections were found as 0.6 µg mL⁻¹ for PAR and KAF and 0.8 µg mL⁻¹ for PRO. According to the validation study, it was proved that the developed method was accurate, precise, sensitive, selective, rugged and robust. Three pharmaceutical preparations, which are produced by different drug companies in Turkey, were analyzed by the developed method. One of the same preparations was also analyzed by the derivative ratio spectrophotometric method reported in literature. No significant differences were found statistically between the results obtained from developed method and the method reported in literature.