Synthesis and characterization of Si-modified quince seed mucilage based bioscaffolds for bone tissue engineering.


Yılmaz H. D., Cengiz U., Arslan Y. E.

III. International Joint Science Congress of Materials and Polymers, Priştine, Kosovo, 12 - 14 September 2019, pp.57

  • Publication Type: Conference Paper / Summary Text
  • City: Priştine
  • Country: Kosovo
  • Page Numbers: pp.57
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

In the field of bone tissue engineering, natural hydrogels are the crucial players due to their biodegradable, bioactive, and bio-mimicking features. Although there are many natural hydrogels, quince seed mucilage (QSM), which is a well-known polysaccharide, was used to construct an osteoregenerative bioscaffolds because of its remarkable wound healing and regenerative capacity. The bioactive and biodegradable properties of the mucilage were enriched with silica molecules to mimic better the nature of bone structure and boost bone regeneration. Silicon modification of the QSM was fulfilled using tetraethyl orthosilicate (TEOS) with sol-gel technique, which is a relatively inexpensive, non-toxic, and well-known way that offers synthesis state-of-theart hybrid gels under mild conditions. The morphological structure of the Si-modified QSM bioscaffolds was investigated under a stereomicroscope, and the presence of silica molecules was proven by energy dispersive spectroscopy analysis in addition to scanning electron microscopy micrographs. The thermal features of the prepared bioscaffolds were determined by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The morphological analyses indicated that Si-modified QSM bioscaffolds possessed interconnected 3D networks, which is required for cell hosting and growth. The average pore size of the Simodified QSM scaffolds was measured to be 66.8 µm. With respect to TGA analysis, it was seen that 80.55% of the total mass was lost at 328.72°C. Also, DSC analysis implied that there is a Td (degradation) point around ~300°C, which supports the TGA data. In conclusion, the mucilage from quince seed, and the silica molecules from TEOS has been successfully reacted to create a durable and highly porous hydrogel. We believe that the novel hybrid material could be used in further cell culture studies, especially as an osteoregenerative material for bone tissue engineering applications.