AQUACULTURE, vol.233, pp.477-493, 2004 (SCI-Expanded)
High nutrient loss due to leakage from microparticulate diets is an important problem in the development of artificial diets for marine fish larvae. Lipid spray beads (LSB) are promising as they can be used to deliver low-molecular weight, water-soluble nutrients. Here, we describe a simple method for the production of LSB composed of methyl palmitate (MP), a low melting point wax, for delivering riboflavin to first-feeding freshwater fish larvae of zebra fish, Brachydanio rerio, and glowlight tetra, Hemigrammus erythrozonus. Characteristics of LSB, such as inclusion, retention and delivery efficiencies as well as overall leaching patterns over time, were used to compare the performances of LSB prepared with different materials.