A simple and validated micellar electrokinetic chromatographic method is described for the determination of 5-hydroxymethylfurfural (HMF). The method was applied to fresh and processed (heated at 65 degrees C, 85 degrees C and 105 degrees C for 5h and 20h) multifloral and honeydew honeys. Optimum conditions were: 10mmolL(-1) Tris buffer consisting 110mmolL(-1) SDS and 10% (v/v) MeOH at pH 9.10, 20kV of applied potential, 3s of injection time at 5x10(3)Nm(-2), 280nm of wavelength and 25 degrees C of fixed temperature. Mean electrophoretic mobility (m(2)s(-1)V(-1)) of HMF and methyl paraben (IS) were -5.28x10(-5) (RSD of 0.745%) and -2.52x10(-4) (RSD of 0.944%), respectively. The calibration curve was linear in the range of 0.68 mu gmL(-1) (5.40x10(-6)molL(-1)) and 4.05 mu gmL(-1) (3.21x10(-5)molL(-1)) with R=0.9990 for inter-day precision. LOD and LOQ values were 0.007 mu gmL(-1) (5.48x10(-8)molL(-1)) and 0.023 mu gmL(-1) (1.83x10(-7)molL(-1)), respectively, as inter-day precision. The accuracy was very high with recovery values between 94.07 and 97.16%. The developed method was fully validated according to ICH guidelines. The results were compared with those of HPLC method adapted from AOAC method no: 980.23.